Phone: (308) 345-3670 | Fax: (308) 345-7880

Directions for Taking Leaf and Petiole Samples

Use the table below as a guide for taking samples:

  1. A leaf or blade sample should consist of 15 or more sub-samples taken at random throughout the area being sampled. A petiole sample should consist of 25 or more sub-samples taken at random. For potatoes, submit a minimum of 40 petioles per sample.
  2. If a sample is taken from a problem area, obtain a comparison sample from a good area. Soil samples from the problem and good areas are helpful to identify the problem.
  3. Avoid sampling along dusty roads.
  4. Note samples that have received foliar fertilizer applications so the samples can be rinsed before analysis.
  5. Place sample in paper bag and ship to the laboratory with a leaf or petiole sample submittal form. Do not mail samples in plastic bags or other air-tight containers.

 

         Crop                                  Stage of Growth                            Plant Part

FIELD CROPS

Alfalfa, clover

 

 1/10 bloom stage

 

 Mature leaf blades from upper 1/3 of plant

 

Beans (soybeans, field beans)

 

Seedling (up to 12” tall)

 

All above ground portion of plant

 

Prior to or at flowering

Most recent fully matured trifoliate leaves

 

Corn

 

Seedling (up to 12” tall)

 

All above ground portion of plant

 

Prior to tasseling

Most fully developed leaf below whorl

 

Tassel or early silk

Ear leaf

 

Cotton

 

Prior to appearance of first squares

 

Petiole from fully expanded leaf on main stem, usually 3rd or 4th leaf from terminal

 

Grass

 

 

At optimum stage for quality

forage or prior to heading

 

 

Four uppermost leaves

 

Small grain – wheat, barley, oats, rye, triticale

Seedling prior to jointing

Prior to heading

All above ground portion of plant

Four uppermost mature leaves

 

Sorghum/milo

 

Seedling (up to 12” tall)

 

Whole plant

 

 

Prior to heading

Soft Dough

 

Fully Expanded Leaf

Third Leaf Below Head

 

Sugar beets

Mid-season

Most recent mature leaf without petiole

 

During growing season

Petiole from most recent mature leaf

 

FRUIT AND NUT CROPS

Apple, cherry, peach, pear

 

 

 

Mid-season

 

 

 

Leaves near base of current year’s growth or from spurs

 

Grapes

 

End of bloom period

 

Petioles from leaves adjacent to fruit clusters

 

Lemon, lime

 

Mid-season

 

Mature leaves from last flush or growth on non-fruiting terminals

 

Orange

 

Mid-season

 

Spring cycle leaves, 4 to 7 months old from non-bearing terminals

 

Pecan

 

6 to 8 weeks after bloom

 

Middle pair of leaflets from mid-portion of terminal growth

 

Pistachio

 

6 to 8 weeks after bloom

 

Leaflet from mid-portion of non-bearing branches

 

 

VEGETABLE CROPS

Cantaloupe

 

 

During growing season

 

 

Petiole of 6th leaf from growing tip

 

Cucumber

 

 

Early fruit set

 

 

Petiole of 6th leaf from tip

 

Muskmelon

 

During growing season

 

Petiole of 6th leaf from growing tip

 

Onion

During growing season

Tallest leaf

 

Pepper – chili

 

During growing season

 

Petiole of young, mature leaf

 

During growing season

Blade of young, mature leaf

 

Pepper – sweet

 

During growing season

 

Petiole of young, mature leaf

 

During growing season

Blade of young, mature leaf

 

Potato

 

 

During growing season

 

 

Petiole of 4th leaf from growing tip

 

Pumpkin

 

Early fruit set

 

Petiole of 6th leaf from growing tip

 

Spinach

 

Midgrowth

 

Petiole of young, mature leaf

 

Tomato

During growing season

Petiole of 4th leaf from growing tip

 

 

During growing season

 

Blade of 4th leaf from growing tip

 

Watermelon

 

Early fruit set

 

Petiole of 6th leaf from growing tip

 

 

ORNAMENTALS

Ornamental trees, shrubs

 

 

 

Current year’s growth

 

 

 

Fully developed leaves

 

Turf

During normal growing

Leaf blades